The effect of trehalose administration on the serum expression levels of microRNAs associated with lipid metabolism and autophagy process in patients with myocardial infarction - the post-hoc analysis of the TREAT-IR trial.

Introduction This study aimed to evaluate the TRE-induced changes in serum expression levels of miRNAs associated with lipids metabolism and autophagy process MI patients assess potential protective effects TRE these patients. Material methods post hoc investigation was performed on samples obtained from a pilot randomized, double blind, placebo-controlled clinical trial that recruited 14 men (aged 18-80) who had systemic inflammation. The were randomized 2:1 ratio either (15 g/week, intravenous (IV) ad-ministration) (N=10) or placebo groups (equal volume saline 0.9 %) (N=4) for period 12 weeks. To measure relative miRNA-155, miRNA-221, miRNA-33, SYBR Green qPCR method used. Results miRNA-155 showed significantly higher group (2.772±0.73; P=0.009) when compared group. Also, significant reductions (0.171±0.03; P=0.016), miRNA-221 (0.116±0.07; P=0.013), miRNA-33a (0.076±0.07; P=0.025) observed at end study. Nevertheless, reduction (normalized baseline) expres-sion (FC:0.87±0.20 vs FC:0.18±0.08; (FC:0.73±0.22 FC:0.13±0.08; less than Conclusions IV administration did not reduce miRNA33a as much placebo. Keeping steady state lipoproteins might have MI.